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The samples used for Western blot analysis were at different concentrations: 25, 50, and 100 µg of protein. The image shows a signal corresponding to the molecular weight around 100 kDa that is reported to correspond to transient receptor potential vanilloid 1 (TRPV1) receptor. The signal was also present in the positive control (first column, K+), composed by proteic lysate of rat urinary bladder.
