2^ (delta Ct) differences in PTEN and mir-183 expression at baseline, demonstrating presence of PTENinbothcell lines, andlowmir-183expressioninKLEcells. At 72hoursafter transfectionof these cell lines with mir-183, decreased PTEN protein expression was noted by Western blot. Identical results were shown after transfection with mir-200c (data not shown). Negative controls (mock and scramble) demonstrated no change in PTENexpression.
Cohn. Comprehensive miRNA profiling of surgically staged endometrial cancer. Am J Obstet Gynecol 2010.