Skip to main content
. 2014 Dec 1;33(6):401–408. doi: 10.1089/mab.2014.0043

FIG. 1.

FIG. 1.

Examination of specificity and sensitivity of antibodies. (A) ELISA assays were performed to detect the titer and specificity of ascites from different CREPT hybridoma clones. (B) ELISA assays were performed to detect the difference of ascites from individuals injected with the same CREPT hybridoma. (C) Sandwich ELISA assay was performed, coating with different dilutions of CREPT multiple clonal antibodies. Different concentrations of GST-CREPT proteins were added to detect the sensitivity of the antibody. Minimal concentration was determined as the concentration with no significant difference to base level of BSA. (D) Capture ELISA assay was performed, coating with CREPT MAb 3E10. Different concentrations of GST-CREPT were used to detect the minimal concentration of CREPT protein that the antibody recognized. The results were from three independent repeats and averages with standard errors are presented. *p<0.05; **p<0.01.