Table 1.

Top V, D, and J Regions of 3E10 Heavy and Light Chains Matched with Ig Sequence

	Top V gene match	Top D gene match	Top J gene match	Identity (%)
VH	IGHV10-1*02	IGHD1-2*01	IGHJ3*01	98.7
VK	IGKV8-30*01	–	IGKJ1*01, IGKJ1*02	99.0

After sequencing, we obtained the information of the gene sequence and performed a BLAST analysis. The results showed that the variable regions in IgH (VH) and IgK (VK) of 3E10 shared 98.7% and 99.0% of identity to the original sequences of the recombined variable light chain, suggesting that VH and HK of 3E10 remained less mutated after the maturation.