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TABLE 2.

Comparison of patient 1's, patient 2's, and the homograft valve isolates with the type and clinical reference strains of O. turbata; C. hominis, and CDC group A-3

Characteristic Isolate
O. turbata ATCC 25835T O. turbata clinical reference isolates (n = 4) Patient 1's isolates and six homograft valve isolates (n = 10) C. hominis DSM 9581T C. hominis clinical reference isolate (n = 1) CDC group A-3 clinical reference isolate (n = 1) Patient 2's isolate and one homograft isolate (n = 2)
Pigment on HIA slants in 3 days Yellow Yellow Yellow Pale yellow Pale yellow Pale yellow Pale yellow
Substrate hyphae +a 100b 100 0
Fermentative acid production from l-rhamnose 0 0 + + + 100
Urease production (Christensen agar slant) 75 30 + (−c) + (−c) 0
Gelatin liquefaction (14 days) + 100 100 − (+c) − (+c) 0
Hydrolysis of casein 100 100 0
Resistanced (MIC) to:
    Amikacin (≥ 64 μg/ml) + 75 100 + + + 100
    Ampicillin (≥4 μg/ml) + 100 100 + + + 100
    Cefotaxime (≥64 μg/ml) + 0 100 + 100
    Ciprofloxacin (≥4 μg/ml) 100 100 + 100
    Erythromycin (≥8 μg/ml) 25 100 + 100
    SMX (>32 μg/ml) + 100 100 + + + 100
    TMP-SMX (>2 to 38 μg/ml) 50 100 + + + 100
a

+, positive; −, negative.

b

Percentage of the isolates that tested positive.

c

Funke et al. (6) described C. hominis as urease negative using Christensen broth and gelatin hydrolysis positive using the film strip method.

d

The MIC breakpoints for resistance that were used are those of NCCLS (13).