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The loss of O acetylation on GXM incubated in human serum is detected by EIA. GXM from strain H99 that was incubated for 4 weeks in human serum was measured in EIAs using MAbs that can distinguish the acetylation state of the polysaccharide. (A) EIA using MAb 2D10 as the capture MAb and MAb 18B7 as the detecting MAb. (B) EIA using MAb 21D2 as the capture MAb and MAb 18B7 as the detecting MAb.
