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. 2004 Jun;42(6):2566–2572. doi: 10.1128/JCM.42.6.2566-2572.2004

FIG. 1.

FIG. 1.

Comparison of MGB probes and probes without a minor groove binder in histograms of CT values of serial dilutions of bacterial DNA. (A) Dilution series of DNA from Escherichia coli detected with the VIC-labeled universal probe. MGB probes are more sensitive than probes without a minor groove binder (detection limit, 10−3 versus 10−1 ng of DNA). The trend lines show similar slopes (MGB probe, 4.49; normal probe, 4.09) but different axis intercepts (MGB probe, 13.17; normal probe, 18.91). (B) Mean CT values of a dilution series of DNA from Escherichia coli, Enterococcus durans, and Bacteroides fragilis, detected with FAM-labeled specific probes. The sensitivity of real-time PCR was higher with MGB probes than with probes without a minor groove binder (10−3 versus 10−1 ng of DNA). The trend lines show similar slopes (MGB probe, 4.52; normal probe, 4.63) but different axis intercepts (MGB probe, 9.89; normal probe, 16.32).