TABLE 1.

Detection of serial dilutions of bacterial DNA: comparison of MGB and non-MGB probes

Probe and DNA concn (label)	CTa with probe:		Detection by conventional PCR with probe:
	MGB	Non-MGB	MGB	Non-MGB
Universal probe (VIC)
10−4	≥40	≥40	+	+
10−3	34.990 ± 0.71	≥40	+	+
10−2	31.560 ± 0.33	≥40	+	+
10−1	27.585 ± 0.21	32.020 ± 0.92	+	+
100	21.755 ± 0.50	25.815 ± 0.28	+	+
101	17.335 ± 0.01	21.490 ± 0.06	+	+
Escherichia coli (FAM)
10−4	≥40	≥40	+	+
10−3	31.330 ± 1.78	≥40	+	+
10−2	28.100 ± 0.17	≥40	+	+
10−1	24.760 ± 2.01	26.263 ± 0.46	+	+
100	20.715 ± 0.18	24.878 ± 0.19	+	+
101	16.925 ± 0.28	21.043 ± 0.29	+	+
Enterococcus durans (FAM)
10−4	≥40	≥40	+	+
10−3	29.530 ± 1.03	≥40	+	+
10−2	24.455 ± 2.01	≥40	+	+
10−1	21.265 ± 0.05	29.417 ± 2.41	+	+
100	17.390 ± 0.13	26.628 ± 2.48	+	+
101	14.660 ± 0.13	20.457 ± 0.56	+	+
Bacteroides fragilis (FAM)
10−4	≥40	≥40	+	+
10−3	33.185 ± 0.23	≥40	+	+
10−2	28.930 ± 0.18	34.257 ± 1.90	+	+
10−1	23.985 ± 0.57	28.937 ± 0.07	+	+
100	18.915 ± 0.23	22.767 ± 0.42	+	+
101	15.650 ± 1.58	18.084 ± 0.50	+	+

^aMean value of two independent experiments ± standard deviation.