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. 2015 Jan;145(1):47–60. doi: 10.1085/jgp.201411272

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© 2015 Yeh et al.

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Figure 5. — Effects of NO₃⁻ on single-channel kinetics of ΔR-CFTR and D1370N/ΔR-CFTR. (A) Modulation of ΔR-CFTR gating by NO₃⁻. Because the activity of ΔR-CFTR is independent of phosphorylation, only ATP is included in the bath. (B) Summary of the effect of NO₃⁻ on the single-channel kinetics of ΔR-CFTR. P_o, τ_o, and τ_c: 0.44 ± 0.02, 342 ± 28 ms, and 432 ± 39 ms for Cl⁻; and 0.68 ± 0.02, 751 ± 138 ms, and 336 ± 55 ms for NO₃⁻ (n = 15). (C) NO₃⁻ increases the P_o of a hydrolysis-deficient mutant CFTR (D1370N). (D) Microscopic kinetic parameters for D1370N/ΔR-CFTR in the presence of bath Cl⁻ or NO₃⁻. P_o, τ_o, and τ_c: 0.41 ± 0.02, 544 ± 45 ms, and 759 ± 48 ms for Cl⁻; and 0.77 ± 0.02, 1,889 ± 278 ms, and 508 ± 71 ms for NO₃⁻ (n = 10). The error bars represent the SEM of the mean.