TABLE 2.
M. avium subsp. paratuberculosis-specific genes in this study
| ORF | Gene identification no. | Size (bp) | Functional domain(s) | Cloneda | Expressedb |
|---|---|---|---|---|---|
| ISMAP02 | 2717932 | 1,674 | None | pBAD, pMal | |
| 2717466 | |||||
| 2719200 | |||||
| 2718191 | |||||
| 2718683 | |||||
| 2720300 | |||||
| MAP0855 | 2720245 | 945 | ATP binding, ATPase | pBAD, pMal | pBAD, pMal |
| 177-31c | 357 | None | pBAD | ||
| MAP0858 | 2718672 | 549 | None | ||
| MAP0860c | 2719015 | 891 | None | pBAD, pMal | pBAD, pMal |
| MAP0862 | 2718315 | 1,083 | None | pBAD | pBAD |
| MAP3732c | 2718816 | 714 | None | pBAD, pMal | pBAD |
| MAP3736c | 2719217 | 960 | Aldolase | pBAD, pMal | |
| MAP1345 | 2718344 | 600 | Methylase | pBAD, pMal | pMal |
| MAP2149c | 2717464 | 645 | None | pMal | |
| MAP2154c | 2717876 | 576 | Protein kinase | pBAD, pMal | pBAD |
| MAP2963c | 2720464 | 2,625 | Heme binding | pMal | pMal |
| MAP3817c | 2718568 | 939 | Thiamine biosynthesis | pBAD, pMal | pMal |
| MAP2756c | 2718333 | 4,911 | None | pBAD, pMal | pBAD, pMal |
a
Indicates which expression vector the sequence was successfully cloned into.
b
Absence of data means that no protein was produced despite successful cloning in an expression vector.
c
This ORF was later found to be a reverse complement truncation of MAP0858