TABLE 5.
Agreement between techniques for related-origin isolatesa
| Isolate pair | Epidemiological relatedness | Classification by technique
|
|||
|---|---|---|---|---|---|
| RAPD | MLEE | Ca3 | MLST | ||
| FC-1 and FC-2 | Switch phenotypes of laboratory strain 3153A | Id | Id | Id | Id |
| FC-3 and FC-4 | Switch phenotypes of laboratory strain WO-1 | Id | Id | Id | Id |
| FC-11 and FC-12 | Same patient, distinct sites of isolation (i.e., oral and fecal) | Id | Id | NId | Id |
| FC-13 and FC-14 | Same patient, distinct sites of isolation (i.e., seminal and oral) | NId | NId | NId | NId |
| FC-17 and FC-18 | Sexual partners, distinct sites of isolation (i.e., vaginal and fecal) | NId | NId | Id | Id |
| FC-19 and FC-20 | Same patient, both vaginal isolates | NId | Id | NId | NId |
| FC-23 and FC-24 | Sexual partners, distinct site of isolation (i.e., vulval and oral) | Id | Id | NId | NId |
a
The 14 isolates (seven pairs) are a subset of the 29 C. albicans isolates (18). Each pair was classified as identical (Id) if both isolates shared the same genotype and nonidentical (NId) if both isolates did not share the same genotype. The genotypes for RAPD analysis, MLEE, and Ca3 Southern hybridization (Ca3) were based on the most discriminatory level of their dendrograms (18); genotypes for MLST were based on DSTs.