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Keratin expression profiles of EPC1 3D cultures with activated fetal esophageal fibroblasts. IHC staining of 3D cultures demonstrates no change in staining for KRT1 (A–D), KRT5 (I–L), or KRT8 (M–P) between any conditions. EPC1-PE cultures have decreased expression levels of KRT4 (E–H) and KRT10 (Q–T) and loss of KRT15 (U–X), compared with EPC1-hTERT control 3D cultures. Scale bar = 50 μm.
