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. 2015 Jan;185(1):96–109. doi: 10.1016/j.ajpath.2014.09.018

Figure 10.

Figure 10

Actin-rich network assembly after reconstitution of ANKRD1 in Ankrd1-deficient fibroblasts. Collagen lattices were loaded with equal numbers of FLOX or knockout (KO) fibroblasts and infected with either adenoviral luciferase–green fluorescent protein (GFP; adLuc-GFP) or ANKRD1-GFP (adAnkrd1-GFP) expression vectors. Cells were stained with phalloidin-red (actin) and SYTOX green (nuclei). Fibrillar actin networks are prominent in collagen gels populated with FLOX fibroblasts infected with adLuc-GFP (ad-Luc) and are more abundant after ANKRD1 overexpression with adAnkrd1-GFP (ad-Ankrd1). Actin fibers are nearly absent in KO cells treated with adLuc-GFP and markedly increase by ANKRD1 overexpression. Insets: The degree of fibroblast-populated collagen lattice contraction, as illustrated in Figure 9. Collaged lattices populated by KO fibroblasts had a much lower cell density because of absent (adLuc-GFP) or reduced (adAnkrd1-GFP) contraction.