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. 2015 Jan;185(1):96–109. doi: 10.1016/j.ajpath.2014.09.018

Figure 9.

Figure 9

Reconstitution of ANKRD1 in Ankrd1−/− fibroblasts restores their ability to contract collagen lattices. A: Western blot analysis of FLOX fibroblasts infected with adLuc–green fluorescent protein (GFP; Luc) and adAnkrd1-GFP (Ankrd1) show overexpression of ANKRD1 after infection with adAnkrd1-GFP. ANKRD1 protein is absent in extracts from knockout (KO) fibroblasts infected with adLuc-GFP but is easily detectable after infection with adAnkrd1-GFP. Cyclophilin A was used as a loading control. B: Gel contraction (dashed circles) accelerates by overexpression of ANKRD1 in FLOX fibroblasts at days 1 and 3; reconstitution of ANKRD1 in KO fibroblasts is significantly restored. KO fibroblast contraction at all time points. ∗∗P < 0.01 (FLOX group), ∗∗∗P < 0.001 (KO group), multiple t-tests.