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. 2015 Jan;185(1):197–213. doi: 10.1016/j.ajpath.2014.09.002

Figure 4.

Figure 4

Craniofacial defects in conditional Porcn-mutant embryos. Gross (A–E) and histological (F–J) images of control and mutant embryos at E15.5. Hematoxylin and eosin stain. A: Frontal view of WT embryo. B:Porcnlox/Y;Wnt1-Cre embryos have a mild cleft in the medial region of the upper lip (arrow). C: Porcnlox/Y;Rx3-Cre embryos have cleft lips (arrow). D:Porcnlox/Y;Wnt1-Cre;Rx3-Cre embryos exhibit craniofacial abnormalities including severe cleft lips (arrow). E: Normal craniofacial development in Porcnlox/+;Wnt1-Cre;Rx3-Cre embryos. F: In control embryos, a uniform palate is formed (arrow). G: In Porcnlox/Y;Wnt1-Cre embryos, the palatal shelves rise horizontally but do not fuse (arrow). H:Porcnlox/Y;Rx3-Cre embryos have cleft palate (arrow). I: In Porcnlox/Y;Wnt1-Cre;Rx3-Cre embryos, the palatal shelves do not rise above the tongue and they fail to fuse (arrow). J: Palatogenesis is normal in Porcnlox/+;Wnt1-Cre;Rx3-Cre embryos (arrow). Scale bar = 500 μm.