. 2015 Jan;185(1):197–213. doi: 10.1016/j.ajpath.2014.09.002

Table 1.

Ocular and Extraocular Abnormalities in Porcn-Mutant Mice at Embryonic Age E12.5 to E18.0

Genotype	OFCD, %	RPE defects,^∗ %			Open eyelid,^† %	Cranio-facial defects, %	MHB defects, %	Normal, %	Total embryos, No.
Genotype	OFCD, %	Severe	Moderate	Mild	Open eyelid,^† %	Cranio-facial defects, %	MHB defects, %	Normal, %	Total embryos, No.
Porcn^lox/Y; Six3-Cre	0	0	0	0	0	0	0	100	19
Porcn^Δ/lox/Y; Six3-Cre	0	0	0	7	13	3	3	90	29
Porcn^het^‡	10	5	18	42	43	28	3	42	60
Porcn^Δ/lox	8	4	31	22	20	35	4	31	26
Porcn^Δ/lox/+; Six3-Cre	12	6	9	53	56	24	3	50	34
Porcn^lox/Y; Wnt1-Cre	2	0	0	0	0	100	100	0	61
Porcn^lox/Y; Rx3-Cre	13	0	20	50	0	95	17	0	30
Porcn^lox/Y; Wnt1-Cre; Rx3-Cre	72	52	69	18	100	100	100	0	29
Porcn^lox/+; Wnt1-Cre; Rx3-Cre	9	0	9	82	0	0	0	22	23
WT	0	0	0	0	0	0	0	100	109

MHB, Mid- and hindbrain; NE, normal; OFCD, optic fissure closure defect; WT, wild-type.

^∗

Severe RPE defects: embryos with transdifferentiated RPE or almost complete loss of pigment (eg, Figure 1E). Moderate RPE defects: eyes with severe pigment gaps or reduction of pigment circumferentially (eg, Figure 2D). Mild RPE defects: eyes with small gaps or a subtle reduction of pigment circumferentially (eg, Figure 2A), analyzed between E15.5 and E18.0.

^†

The eyelid closure defect was determined at E16.5 to E18.0.

^‡

Porcn^Δ/lox and Porcn^Δ/lox;Six3-Cre combined.