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. 2015 Jan 1;11(1):48–58. doi: 10.7150/ijbs.9969

Figure 6.

Figure 6

let-7f did not induce apoptosis of MCF-7 cells. A, The effects of let-7f and AMO-let-7f on apoptosis were evaluated by Annexin V/PI staining using flow cytometry. MCF-7 tumor cells were treated with let-7f, AMO-let-7f or NC siRNA for 24 hours. Lower right quadrant (Q4) Annexin V positive/PI negative cells denote early apoptosis and upper right quadrant (Q2) Annexin V positive/PI positive cells denote late apoptosis or necrosis cells. Images are the representative of three independent experiments. B, Statistic analysis of the results of apoptosis assay. let-7f induced apoptosis of MCF-7 cells including early apoptosis (P<0.05) and late apoptosis/necrosis cells (P<0.01), while AMO-let-7f did not produce any effects on apoptosis of MCF-7 cells (P>0.05). * P<0.05 vs NC, ** P<0.01 vs NC.