Table 2.
Primer Sequence (5'-3') | ||
---|---|---|
THBS1 | Forward | (SgfI) gaatgcgatcgcTCATCAGCTGCCAATCATAAC |
Reverse | ( XhoI) ccgctcgagTATAAATATAAACTTCCATATGAT | |
mutTHBS1 | Forward | GTGGAACTTCTATACAAATATATGGAGTTTTGTTGTGTGACTGAGTAA |
Reverse | TTACTCAGTCACACAACAAAACTCCATATATTTGTATAGAAGTTCCAC |
Note: SgfI and XhoI are name of restriction enzyme.