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. 2015 Jan 1;11(1):75–87. doi: 10.7150/ijbs.9872

Figure 6.

Figure 6

Binding affinities of the ligands to the SlitOBP1 mutants. (A) Binding affinities of mutant Thr9. (B) Binding affinities of mutant Glu98. (C) Binding affinities of mutant Trp37. Final solutions of 2 μM SlitOBP1 protein and 2 μM 1-NPN were titrated with 1 mM solution of each ligand in methanol to final concentrations of 0-20 μM. The figure reported averages of three replicates. (D) The Ki values of the binding affinities. Values are means of three independent experiments. Ligands concentration > 20μM for half-maximal relative fluorescence intensity was represented as “-“.