Table 3.
Fluorescence competitive binding affinities of selected components to recombinant SlitOBP1.
| Ligands | CAS | SlitOBP1 | |
|---|---|---|---|
| IC50 | Ki | ||
| Alcohols | |||
| 1H,1H,2H,2H-perfluoro-1-decanol | 678-39-7 | 5.97 | 3.69 |
| 1H,1H,10H,10H-perfluoro-1,10-decanediol | 754-96-1 | 6.68 | 4.13 |
| 1H,1H,9H,9H-perfluoro-1,9-nonanediol | 203303-01-9 | 9.45 | 5.84 |
| Acids | |||
| Perfluorononanoic acid | 375-95-1 | 6.08 | 3.76 |
| 9-Chlorohexadecafluorononanoic acid | 865-79-2 | 6.00 | 3.73 |
| H-Lys-Glu-OH | 45234-02-4 | 5.86 | 3.62 |
| H-Glu-Lys-OH | 5891-53-2 | - | - |
| H-Gly-Tyr-Gly-OH | 6099-08-7 | 6.11 | 3.78 |
| H-Phe-Met-OH | 15080-84-9 | - | - |
| H-Lys-Ile-OH | 20556-13-2 | 7.52 | 4.65 |
| H-Tyr-Glu-OH | 2545-89-3 | - | - |
| N-Cbz-L-Aspartic acid 4-tert-butyl ester | 5545-52-8 | 6.70 | 4.14 |
| Ketones | |||
| 1,2-Dehydrocortisone | 53-03-2 | 5.00 | 3.09 |
| Delta-5-pregnenolone | 145-13-1 | 14.77 | 9.13 |
| 11β,17α,21-trihydroxypregna-1,4-diene-3,20-dione | 50-24-8 | 5.02 | 3.10 |
| Heterocyclic compounds | |||
| 16,17-epoxypregnenolone | 974-23-2 | 8.28 | 5.12 |
| Corynoline | 18797-79-0 | - | - |
| Pyr-His-Pro-NH2 | 24305-27-9 | 7.18 | 4.44 |
| L-Tyrosine benzyl ester | 42406-77-9 | 7.23 | 4.47 |
| Others | |||
| Perfluorosebaconitrile | 2342-09-8 | - | - |
| 1H,1H,2H,2H-perfluorodecylamine | 30670-30-5 | - | - |