Figure 3.
Up-regulation of CEP2 mediated by lentivirus inhibited myoblast differentiation. qPCR (A) and (B) western blotting for myogenic factors of CEP2-expressing cells and control cells were detected in GM on the 2nd d. After differentiation for 4 days, the mRNA (C) and protein (D) of myogenic factors were analyzed again. (E) The rate of myogenin expression cells was analyzed with immunofluorescence. Scale bar = 50μm. CON: native control (an empty lentivirus vector); CEP2 (lentivirus-based CEP2). GFP: represents lentivirus infection efficiency.*p<0.05; **p<0.01; n ≥ 3.