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. 2015 Jan 1;11(1):99–108. doi: 10.7150/ijbs.8621

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Knockdown of CEP2 enhanced myogenesis. RNA (A) and protein (B) analysis of myogenic factors after transfection of siCEP2 to C2C12 in GM on the 2^nd d. siLamin A: positive control (siRNA pool targeted to Lamin A). After differentiation for 4 days, the mRNA (C) and protein (D) of myogenic factors was analyzed. The rate of myogenin expression cells (E) and MyHC (F) were detected with immunofluorescence in DM on the 4^th d. Scale bar = 50μm. siCEP2: siRNA pool targeted to CEP2, NC: native control (No target siRNA pool). *p<0.05; n ≥ 3.