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. 2015 Jan 1;11(1):109–121. doi: 10.7150/ijbs.10276

Figure 5.

Figure 5

MiR-467a targets Fas and Bax in thymic lymphoma cells. (A) Predicted wild type (WT) and mutant (MUT) miR-467a binding sites at mouse Fas and Bax mRNA 3'UTR. (B) Interaction of miR-467a with the 3'UTR of Fas and Bax. At 48 h after transfection with miR-467a mimics or miR-NC mimics vector, a reporter plasmid containing Fas or Bax wt-3'UTR or mut-3'UTR and a plasmid expressing renilla luciferase were cotransfected into EL4 cells. Luciferase activities were measured at 48 h after transfection with the plasmids, and normalized data are shown. The data represent mean ± SD of triple determinations. (C) EL or NIH3T3 cells were transfected with miR-467a mimics or miR-NC mimics vector. 24 h later, these cells were exposed to 7-Gy irradiation for 5 min. 24 h after exposure, the protein levels of endogenous Fas and Bax were determined. FACS assay shows that exogenous miR-467a downregulates Fas protein level in EL4 and NIH3T3 cells. (D) Immunoblotting shows that exogenous miR-467a downregulates Fas and Bax protein level in these cells. * p < 0.05. NS, no significant.