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. 2014 Oct 15;1(10):634–648. doi: 10.18632/oncoscience.87

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© 2014 Van Bockstal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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SW480 cells were seeded onto CAF-derived matrices with or without decorin coating, and cell adhesion was measured by MTT (A) and SRB (B) assay after 24 hours. Results are presented from three wells per assay from two independent experiments. Values are mean percentage ± SD. CAFs that produced the matrices were treated either with vehicle control, bFGF, TGF-β1 or both cytokines. (C) When seeded on matrices derived from TGF-β1-treated CAFs, elongated SW480 cells adhered in a highly organized fashion along preformed ‘tracks’ in the matrices, whereas the control or bFGF treatment showed a rather random distribution of the cells. Scale bar: 200 μm.