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. 2014 Oct 5;1(10):624–633. doi: 10.18632/oncoscience.86

Figure 4. PCTAIRE1 knockdown did not diminish HaCaT keratinocyte growth.

Figure 4

(A) HaCaT keratinocytes were transfected with scrambled RNA or two different siRNAs targeting PCTAIRE1 (siRNA#1, #2). Three days after transfection, cell lysates were prepared and analyzed by immunoblotting. (B) HaCaT cells were transfected with siRNAs as indicated. After 3 days, cellular ATP levels were measured using Cell Titer Glo reagents (mean ± SD; n = 3). (C) To measure cell growth, 2.0 × 105 cells transfected with the indicated siRNAs were plated. After 3 days, the number of cells was counted (mean ± SD; n = 3).