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. 2014 Oct 5;1(10):624–633. doi: 10.18632/oncoscience.86

Figure 6. PCTAIRE1 knockdown leads to accumulation of p27.

Figure 6

(A) A2058 cells were transfected with control RNA or two different siRNAs targeting PCTAIRE1. Three days after transfection, cell lysates were prepared and analyzed by immunoblotting. (B) A2058 cells stably containing inducible shRNAs targeting PCTAIRE1 (shRNA#1, #2) were cultured for 3 days with (ON) or without (OFF) 100 ng/ml doxycycline (Dox). Protein lysates were analyzed by SDS-PAGE/immunoblotting. (C) SK-MEL-28 cells with inducible shRNAs (#1, #2) were cultured for 3 days with (ON) or without (OFF) doxycycline (Dox). Protein lysates were analyzed by SDS-PAGE/ immunoblotting. (D) HaCaT keratinocytes were transfected with RNAs as indicated. After 3 days, cell lysates were prepared and analyzed by immunoblotting.