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. 2014 Mar 31;1(3):229–240. doi: 10.18632/oncoscience.27

Figure 5. PIAS1 suppresses MDA-MB-231 breast cancer cell-derived metastasis in mice in vivo.

Figure 5

A. Stable clones of the MDA-MB-231-Luc expressing the SUMO E3 ligase mutant PIAS1 (CS) (blue symbols and line) or transfected with the vector control (black symbols and line) were seeded in a 96-well plate at increasing concentrations and subjected to luciferase assay one day later. Linear regression indicated an R2 of 0.98 for the relationship between cell number and luciferase activity for both control and PIAS1 (CS) clones. B. Representative ventral bioluminescence images of mice taken at 10, 14, 17 and 21 days post intracardiac injection of vector control or PIAS1 (CS) stably expressing MDA-MB-231-Luc breast cancer cells. C. Whole body bioluminescence for the control and PIAS1 (CS) expressing breast cancer cells. D. Knee bone metastasis analysis. *, or ***, refers to P < 0.05, or P< 0.001, respectively (ANOVA).