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. 2014 May 6;1(5):336–348. doi: 10.18632/oncoscience.36

Figure 3. Elevated levels of Spy1 protein promote neuroblastoma progenitor self renewal.

Figure 3

(A) SH-SY5Y cells cultured as neurospheres (NS) or in monolayer (ML) were harvested and SPDYA expression levels determined by qRT-PCR. Data shown is mean ±s.d, ***p < 0.001. (Student's t-test, n=3). (B-D) SH-SY5Y-WT and SH-SY5Y-Spy1 cells were grown in neurosphere assays. (B) Morphology of cultures by light microscopy (left panels). Scale bar, 100 μm. Spheres were passaged every 6-7 days and the neurosphere formation efficiency was quantified as a number of spheres relative to the total number of the cells seeded (right panel). Representative data are shown as mean ±s.d. of triplicates from three independent experiments, **p < 0.01 (Student's t-test; n=3). (C) Neurospheres maintained in culture for 14 days were scored according to diameter. Values presented as mean ±s.d. of two independent experiments, **p < 0.01. (Student's t-test). (D) Primary, secondary and tertiary neurospheres were subjected to MTT assay. The obtained absorbance at 590nm was corrected for background absorbance. Data shown is mean ±s.d, *p < 0.05 (Student's t-test; n=2).