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. 2014 May 6;1(5):336–348. doi: 10.18632/oncoscience.36

Figure 6. Clonal properties of CD133+ populations of neuroblastoma depend on Spy1.

Figure 6

(A) CHLA-15 cells sorted for CD133. Expression levels of CD133 depicted in CD133+ vs. CD133- population. (B) CD133- and CD133+ populations analyzed for the number and size of spheres formed. (C; left) Spy1 mRNA levels in CD133- cells subjected to Spy1 overexpression. (C; right) CD133+ population subjected to knockdown control (pLKO-scrambled) or Spy1 knockdown (pLKO-shSpy1 #1 and #2). (D) Number and size of tumorspheres formed in CD133- cells overexpressing Spy1 (left) or CD133+ Spy1 knockdown cells (right). (E) CD133- population overexpressing Spy1 assessed for expression of CD133, Ki67 or c-MYC. All genes are expressed relative to GAPDH and pEIZ-Control(cntl) genes are normalized to 1. pEIZ-Spy1 expression is shown relative to pEIZ-Control. (F) Ki67 (left) and MYC (right) expression in CD133+ cells expressing pLKO-shSpy1#1, pLKO-shSpy1#2 or pLKO-scrambled. (A-F) Data is expressed as mean ±s.d. of triplicates from three independent experiments, *p < 0.05, **p < 0.01, ***p<0.001 (Student's t-test).