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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Neurotoxicology. 2014 Aug 10;44:303–313. doi: 10.1016/j.neuro.2014.08.001

Figure 7.

Figure 7

Quantitative infrared immunofluorescence analysis of striatal TH levels. Coronal slices from rat STR were probed for antibodies against TH. Secondary antibodies were tagged with an infrared fluorescent marker. Depicted are representative scans showing fluorescence intensity in STR slices from control animals (A) and Mn treated animals (B). Manually selected ROIs are shown as dashed lines. A minimum of three slices were quantified from each animal and the resulting values were averaged. Group values were averaged and are shown (C). No difference was observed between control and either low or high dose groups. Mn treatment did not affect STR TH signal intensity. ANOVA with Tukey’s post hoc test for multiple comparisons. Data represent mean ± SD (n = 4–5).