Skip to main content
. 2004 Jun;42(6):2403–2410. doi: 10.1128/JCM.42.6.2403-2410.2004

FIG. 2.

FIG. 2.

LDR products for all combinations of the four human Plasmodium parasite species: P. vivax (Pv), P. falciparum (Pf), P. malariae (Pm), and P. ovale (Po). In step one of the PCR-LDR assay, PCR products for all possible combinations of the four human malaria parasite species were amplified from cloned plasmid-based templates. In step two of the PCR-LDR assay, the PCR products were used as templates for the multiplex species-specific LDR. Input templates for each lane were as follows: lane 1, P. vivax and P. falciparum; lane 2, P. falciparum and P. malariae; lane 3, P. falciparum and P. ovale; lane 4, P. vivax and P. malariae; lane 5, P. vivax and P. ovale; lane 6, P. malariae and P. ovale; lane 7, P. vivax, P. falciparum, and P. malariae; lane 8, P. vivax, P. falciparum, and P. ovale; lane 9, P. falciparum, P. malariae, and P. ovale; lane 10, P. vivax, P. malariae, and P. ovale; lane 11, P. vivax, P. falciparum, P. malariae, and P. ovale; lane 12, P. falciparum; lane 13, P. vivax; lane 14, P. malariae; lane 15, P. ovale. The LDR products identified on the right were 63 nucleotides (P. vivax), 60 nucleotides (P. falciparum), 57 nucleotides (P. malariae), and 54 nucleotides (P. ovale).