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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Dev Dyn. 2014 Oct 18;243(12):1571–1580. doi: 10.1002/dvdy.24191

Figure 4. osr1 is a direct target of Nodal signaling.

Figure 4

(A, B, C, D) animal pole view; (A’, B’, C’, D’) lateral view, dorsal to the right. 4 hpf embryos were injected with 2 ng recombinant mouse Nodal protein (B, B’) or 400 ng BSA (A, A’). Half of the injected embryos were treated with 50µg/ml cycloheximide (C, C’, D, D’), and all were fixed after 1 hr and 20 minutes. Nodal protein strongly induces ectopic osr1 expression in the animal pole of the embryo (B, B’). Ectopic expression is induced in the presence of cycloheximide indicating that osr1 is a direct target of Nodal signaling (D, D’).