Figure 6. Loss of osr1 increases the number of sox32 expressing endoderm cells.

sox32 expressing cells were counted in osr1 morphants and embryos injected with a control morpholino. (A) and (B) show lateral views of control and osr1 morphant embryos, respectively (dorsal is on the right). Cells were counted in identical sized windows (area = 0.05mm²) (A', B') on both lateral sides and the dorsal side (shield). Average number of cells per 0.05mm² on each side is shown in (C). Welch's t-test analysis indicates that osr1 morphant embryos show a significant increase in the number of sox32 expressing cells (D). n=7 (control); n=10 (osr1 MO). Scale bar in (A', B') = 0.1mm.