Figure 1.
Sec2p is associated with polyA+ RNA and is more strongly associated in hyphae compared to yeast.
A. Strains expressing Sec2p–YFP or control Gin4p–GFP as indicated were used in an RNA immune precipitation experiment (RIP). Any mRNA that was present in the immune precipitates was used as a template for RT PCR using either polydT (18) or randomized polyN6 primers, 32-P dCTP and Superscipt III reverse transcriptase as described in Experimental procedures.
B. An RIP experiment using lysates from Yeast YFP expressed from the constitutive PGK1 promoter as a negative control and randomized Poly-(dT)18 as primers.