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. 2014 Oct 14;94(4):828–842. doi: 10.1111/mmi.12799

Figure 2.

Figure 2

Sec2p–GFP is specifically associated with SEC2 mRNA.

A. The top 16 hits from a microarray analysis of the Sec2p-3xFLAG RIP experiment. The data are presented as the ratio of the indicated RNA to the control Gin4p-3xFLAG RIP.

B. Verification of the microarray data for SEC2 RNA and representative genes shown in panel A. qRT-PCR was performed using templates for the indicated genes when the lysis buffer contained either 1 mM EDTA or 10 mM EDTA to remove magnesium ions generally required for protein–RNA interactions. The gene names are shown in abbreviated form with the orf19 prefix not shown. The ordinate shows the fold increase relative to control Gin4p-3xFLAG RIP.