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. 2014 Oct 14;94(4):828–842. doi: 10.1111/mmi.12799

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© 2014 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Sec2p is not associated with mRNAs transported by She3p.A.

A reciprocal immune precipitation experiment showing a lack of detectable association between She3p and Sec2p. Sec2p-HA or She3p–GFP were immune precipitated as indicated (IP). The immune precipitates were used in a Western blot experiment with monoclonal antibodies to GFP or HA as indicated.

B. RIP experiments were carried out with She3p-3xFLAG, Sec2p-3xFLAG and Gin4p-3xFLAG. RT-qPCR was carried out using primers to ACT1, SAP5 or RBT4. The chart shows the enrichment of the indicated RNAs in each immune precipitate relative to the Gin4p-3xFLAG control.