FIG 3 .

Complementation of the growth defect of the S. cerevisiae tps1 tps2 strain on fructose by R. solanacearum ripTPS. RipTPS with or without an HA tag was expressed in the tps1 tps2 yeast mutant, and the growth of the strain was compared to that of strains carrying an empty vector or expressing E. coli OtsA with an HA tag or one of the three catalytic mutants of RipTPS (Y154V, W163S, and D208G) with an HA tag. Amounts of 5 µl of liquid cultures were spotted as a series of 10-times dilutions on 2% galactose or 2% fructose SD-LWU plates. The control yeast strain (WT) was grown on plates with LWU. Pictures of a representative experiment at day 2 are shown.