FIG 5 .
ADP-ribosylation of NLRP3 by CARDS toxin. (A) Cell lysates prepared from HEK 293 cells expressing GFP-NLRP3 were incubated with CARDS toxin (+) (140 pmol) or without CARDS toxin (−) in the presence of 32P-NAD. Then, radiolabeled lysates were immunoprecipitated with anti-GFP antibodies, resolved by Nu-PAGE, transferred to nitrocellulose membranes, and exposed to X-ray films. (B) The same membrane exposed to X-ray film (as shown in panel A) was immunoblotted with anti-NLRP3 antibody to visualize the NLRP3 (i.e., GFP-NLRP3) protein band.