Table 2.

Recognition of recombinant group 5 or 6 allergens and versions of the C-terminal domain of Phl p 5.0201 in a direct binding assay

Clone	Recombinant protein
	Phl p 1 (negative control)	Phl p 5.0101	Phl p 5.0201	Phl p 6.0101	Phl p 5.0201 C-terminal domain*,†
					Wild-type	Mutations 1-6	Mutations 1 + 10	Mutations 1-6, 10	Mutations 7-10
4.3	−‡	+++	++	+++	−	−	−	−	−
4.12	−	+++	++	−	−	−	−	++	−
p5-MA5	−	+++	+++	−	++	+++	+	+++	−
p5-AB5	−	+++	+++	−	+	+++	++	+++	−

^*The following mutations in the C-terminal domain of Phl p 5.0201 were investigated: mutation number 1: K149A; 2: K160A; 3: K166A; 4: K190A; 5: K193A; 6: K204A; 7: K214A; 8: K238A; 9: K248A; 10: K283A.

^†Residues 214, 238 and 248 (modified by mutations 7-9) in Phl p 5.0201 correspond to residues 246, 270 and 280 in Phl p 5.0101 (Figure2A).

^‡Absorbance values: −: A₄₅₀ = 0–0.5; +: A₄₅₀ = 0.5–1; ++: A₄₅₀ = 1–2; +++: A₄₅₀ = 2–3.