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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Sep 26;453(3):326–331. doi: 10.1016/j.bbrc.2014.09.068

Figure 3. Inflammatory cytokines, IL-1β and TNF-α, significantly increase levels of CRYAB in U373 cells and also its secretion of via exosomes.

Figure 3

(A) Exposure of U373 cells to inflammatory cytokines, IL-1β and TNF-α, for 24h showed a significant increase (B) in CRYAB levels compared to untreated cells. β-actin was used as loading control. Error bars, SD (*p<0.05). Similarly exosomes isolated from U373 cells exposed to inflammatory cytokines, IL-1β or TNF-α, were analyzed by western blot (C) for CRYAB levels. (D) Quantification of the western blots showed a significant increase in levels of secreted CRYAB in exosomes isolated from IL-1β and TNF-α treated cells. β-actin was used as loading control. Error bars, SD (p<0.0005).