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. Author manuscript; available in PMC: 2015 Apr 3.

Published in final edited form as: J Mol Biol. 2013 Dec 12;426(7):1390–1406. doi: 10.1016/j.jmb.2013.11.031

Table 1.

ETV6 constructs and DNA sequences

Name	Sequence	Comment
ETV6^R426	G329-R426	Uninhibited ETV6^a

ETV6^D446	G329-D446	Inhibited ETV6^a

ETV6^D446	R335-D446	Inhibited ETV6^a

ETV6^R458	R335-R458	Inhibited ETV6^a

DNA^sp	5′-CAAGCCGGAAGTGAG-3′	Specific DNA^b
DNA^sp	3′-GTTCGGCCTTCACTC-5′	Specific DNA^b
DNA^sp-cryst	5′-AAAGCCGGAAGTGAG-3′	Specific DNA^b
DNA^sp-cryst	3′-TTCGGCCTTCACTCT-5′	Specific DNA^b
DNA^nonsp	5′-GATGCAGTGTAGTCG-3′	Non-specific DNA
DNA^nonsp	3′-CTACGTCACATCAGC-5′	Non-specific DNA
DNA^sp-emsa-1	5′-CAAGCCGGAAGTGAG-3′	Specific DNA^b
DNA^sp-emsa-1	3′-GTTCGGCCTTCACTC-5′	Specific DNA^b
DNA^sp-emsa-2	5′-CAAGCCGGATGTGAG-3′	Specific DNA^b
DNA^sp-emsa-2	3′-GTTCGGCCTACACTC-5′	Specific DNA^b

^a

The core ETS domain spans Leu337 to Phe415, and Pro419 to Ser424 form helix H4.

^b

The core ^5′GGAA^3′ or ^5′GGAT^3′ binding motif is in bold.