Figure 2.

Profile of leukotriene C₄ (LTC₄) produced by isolated rat lungs perfused with buffer alone or with erythrocytes, with metabolites measured at 15 minutes of perfusion. Three separate groups of lungs were perfused with buffer (GPBS; n = 6), normal erythrocytes (HbAA-RBC; n = 6), or sickle cell erythrocytes (HbSS-RBC; n = 12). Perfusates were collected as described in “Methods,” and the LTC₄ was extracted, purified by high-pressure liquid chromatography, and measured by enzyme-linked immunosorbent assay. Data are means + SEM. A single asterisk indicates significant (P < 0.05) difference from LTC₄ production in GPBS control; double asterisks indicate significant (P < 0.05) difference from both GPBS controls and the HbAA-RBC group. GPBS: phosphate-buffered saline containing 131 mM NaCl, 5.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, 1% bovine serum albumin, and 8.3 mM glucose.