TABLE 2.

DNA sequencing and real-time PCR data for rpoB mutations in Rmp^r M. tuberculosis strains from Poland

Affected codon	Nucleotide change(s)	Amino acid change	No. (%) of strains with mutation	Deviation of Tm (°C) from that for wta
				RPO1 probe (65.4°C)	rpo probe (63.2°C)
531	TCG→TTG	Ser→Leu	24 (37.5)		+1.92 to +2.62
	TCG→TGG	Ser→Trp	2 (3.1)		ND
526	CAC→ACC	His→Thr	2 (3.1)		−1.78
	CAC→GAC	His→Asp	2 (3.1)		−3.08 to −3.38
	CAC→TGC	His→Cys	1 (1.6)		ND
	CAC→TAC	His→Tyr	1 (1.6)		−1.78
522	TCG→TTG	Ser→Leu	1 (1.6)	ND
517	CAA→CTAb	Gln→Leu	1 (1.6)	−3.83
516	GAC→TAC	Asp→Tyr	6 (9.4)	−2.33 to −2.83
	GAC→GTC	Asp→Val	3 (4.7)	−2.53
	GAC→GTGb	Asp→Val	1 (1.6)	−2.83
513	CAA→CTA	Gln→Leu	2 (3.1)	−3.83
	CAA→AAA	Gln→Lys	2 (3.1)	−6.33
514	TTC	Phe (Ins)c	1 (1.6)	−5.33
516 and 529	GAC→GGC and CGA→CAA	Asp→Gly and Arg→Gln	1 (1.6)	ND
515 and 516	ATG→ATTb and GAC→TAC	Met→Ile and Asp→Tyr	8 (12.5)	−6.33 to −6.63
513 and 526	CAA→CTA and CAC→AAC	Gln→Leu and His→Asn	1 (1.6)	ND
512 and 516	AGC→ATCb and GAC→GGC	Ser→Ile and Asp→Gly	1 (1.6)	−7.33
510 and 516	CAG→CACb and GAC→TAC	Gln→His and Asp→Tyr	2 (3.1)	−6.33
None	None	None (wt)	2 (3.1)	−0.13 to −0.17

^aDeviations in the T_ms for the RPO1 (7) and rpo (25) probes in comparison with the value for the reference wt sequence (given in parentheses); ND, not determined.

^bNew allele.

^cIns, insertion.