Table 2.

Reacted heme and alkylated histidine chemical shifts of T111H-A⁴ and L75H-B cyanomet CtrHbs^a

	T111H-A4	L75H-B
1-CH3	2.02	15.57 (−26.3)
2-v α	13.47	26.44
2-v βc, βt	−2.83, −3.84	−5.46, −5.78
3-CH3	20.26	11.55 (−31.1)
4-α	6.13	0.54
4-β-CH3	3.45	−0.49 (25.2)
5-CH3	5.88	18.81 (−34.8)
6-p α, α’	8.50, 1.87	12.91, 12.56
6-p β, β’	−1.03, −1.84	−0.27, −1.57
7-p α, α’	15.46, 12.19	7.74, 3.89
7-p β, β’	0.53, −0.81	−1.75, −2.66
8-CH3	18.65	5.66 (−12.7)
H111 Hε1, Hδ2	8.36, 6.48	n/a
H111 Nδ1, Nε2	250.6, 220.7	n/a
H75 Hε1, Hδ2	n/a	7.12, 5.42
H75 Nδ1, Nε2	n/a	254.6, 198.6

^apH 7.2, 298 K.

Values in parentheses are ¹³C shifts.