Table 1. Diffusion Parameters of Syt7 C2 Species.
species | D (μm2/s)a | major diffusing population (%)a,b | fast-diffusing population (%)a,b | n |
---|---|---|---|---|
C2A | 1.82 ± 0.12 | 10 | ||
C2B | 1.82 ± 0.08 | 14 | ||
C2AB-WT | 0.99 ± 0.06 | 83 ± 5 | 15 ± 4 | 8 |
C2AB-FE4 | 1.03 ± 0.07 | 87 ± 6 | 11 ± 6 | 9 |
C2AB-FE16 | 1.00 ± 0.07 | 93 ± 2 | 5 ± 2 | 8 |
Dpred | 0.91 ± 0.08c |
Mean ± 95% CI among n independent samples (supported bilayers) are reported.
From two-component fits to C2AB tandem domain species. D from the major component is reported here. Populations total less than 100% due to the presence of a small population (∼2%) of immobile particles.
Dpred was calculated using eq 2 and propagating error from DC2A and DC2B.