FIG. 1.

Schematic representation of the C. trachomatis genome. The omp1 gene, approximately 1.2 kb in length, was PCR amplified with various combinations of primers. Primers P1 and OMP2 were used in the primary PCR. DNA which failed to generate primary PCR products was subsequently amplified in a nested PCR with the following primer combinations: P1-CT6R, CT6F-OMP2, and NL-F-NL-R. Nucleotide sequence data from the C. trachomatis conjunctival samples was assembled into one contiguous sequence spanning the four variable domains (VDI to VDIV).