Figure 4. CFC assay after 12 days of culture.
(A) MuMac-E8 cells were cultured for 12 days in methylcellulose-based semi-solid medium. The initial cell number was 2×105 cells (magnification 100-fold). Cells revealed a distinct colony formation. The colonies consist of numerous cells of predominantly round shape. By comparison with example images from the manufacturer's instructions MuMac-E8 colonies were identified as CFU-M. (B) May-Grünwald-Giemsa stained MuMac-E8 cells harvested from bulk culture and allowed to adhere in chamber slides.