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. 2014 Dec 29;9(12):e113743. doi: 10.1371/journal.pone.0113743

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© 2014 Fricke et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MuMac-E8 cells were stained with fluorochrome-labeled monoclonal antibodies recognizing the surface marker proteins CD11b, F4/80 (A), CD14, and CD64 (B) in order to identify the functional phenotype of this cell line. It could be shown that MuMac-E8 cells in general express CD11b and that the majority of cultured MuMac-E8 cells express also F4/80 (91%). The number of CD11b⁺/F4/80⁺ cells increased following co-incubation with heat-killed S.E. (96%; A, right-hand panel). CD11b⁺/F4/80⁺ MuMac-E8 cells revealed high or moderate surface expression of CD14 or CD64, respectively. Moreover, the expression of both CD14 and CD64 on CD11b⁺/F4/80⁺ MuMac-E8 cells was additionally inducible by stimulation with Salmonella antigen (B).