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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Acta Biomater. 2014 Sep 19;11:37–47. doi: 10.1016/j.actbio.2014.09.022

Figure 6.

Figure 6

Inhibition of NFκB reduces IL-4-induced fusion. Representative images of WT macrophages treated with IL-4 for 3 d (A, B) or 5 d (C, D) in the presence of BAY 11 (B, D) and stained with anti-RelA (A, B) or Giemsa and May Grunwald (C, D) are shown. RelA immunoreactivity was detected with FITC-conjugated secondary Ab. Cytoskeleton and nuclei were stained with rhodamine-phalloidin and DAPI, respectively. Scale bar = 50 μm. (E) Quantification of fusion was performed from randomly selected (10 per well) in triplicate wells from three independent experiments. Values represent mean + SEM, n = 3. ** * indicates p ≤ 0.0001.