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. 2014 Dec 29;9(12):e115901. doi: 10.1371/journal.pone.0115901

Figure 3. Effect of overexpression of Fis on the level of transcription from the lapF promoter.

Figure 3

β-Galactosidase (β-Gal) activity expressed from the lapF promoter-lacZ reporter constructs were measured in the wild-type strain PSm and the Fis-overexpressing strain F15 of P. putida grown for 18 hours in LB medium supplemented with 1 mM IPTG and without IPTG supplementation. Vertical bars denote 95% confidence intervals of means. Data of at least 8 independent measurements is shown. The length of the lapF promoter region inserted upstream of the lacZ reporter gene in different pBLKT constructs is shown above diagrams. The Fis-binding site Fis-F2 (gray box) and 5′ end of lapF mRNA F-I (arrow) are shown in drawings.